本抗原清单共涉及145种抗原肽，来自21篇2005年至2024年的研究文献。该清单可用于结肠癌肿瘤疫苗的研发测试，也可以用于结肠癌T细胞疗法的靶点选择。为能够检验抗原肽的T细胞活化特性，针对每种抗原肽HLA，我们都开发了四聚体，以用于疫苗活化T细胞的效果测试。
本抗原清单来源的文献名称
| 文献题目 | 期刊名称 | 发表时间 | 抗原数量 |
| An analogue peptide from the Cancer/Testis antigen PASD1 induces CD8+ T cell responses against naturally processed peptide. | Cancer Immun | 2013 | 1 |
| Anti-cancer immune effect of human colorectal cancer neoantigen peptide based on MHC class I molecular affinity screening. | Front Immunol | 2024 | 82 |
| Antigen Identification for Orphan T Cell Receptors Expressed on Tumor-Infiltrating Lymphocytes. | Cell | 2018 | 26 |
| Antigen-delivery through invariant chain (CD74) boosts CD8 and CD4 T cell immunity. | Oncoimmunology | 2019 | 2 |
| Arginase-1 targeting peptide vaccine in patients with metastatic solid tumors – A phase I trial. | Front Immunol | 2022 | 3 |
| Dendritic cell-based immunotherapy for colon cancer using an HLA-A*0201-restricted cytotoxic T-lymphocyte epitope from tumor-associated antigen 90K. | Cell Mol Immunol | 2013 | 3 |
| Enhanced anti-colon cancer immune responses with modified eEF2-derived peptides. | Cancer Lett | 2015 | 3 |
| First clinical trial of cancer vaccine therapy with artificially synthesized helper/ killer-hybrid epitope long peptide of MAGE-A4 cancer antigen. | Cancer Sci | 2012 | 1 |
| Heat shock protein 90 targets a chaperoned peptide to the static early endosome for efficient cross-presentation by human dendritic cells. | Cancer Sci | 2015 | 1 |
| Identification and characterization of agonist epitopes of the MUC1-C oncoprotein. | Cancer Immunol Immunother | 2014 | 10 |
| Identification and Validation of T-cell Receptors Targeting Hotspot Mutations in Human Cancers for Use in Cell-based Immunotherapy. | Clin Cancer Res | 2021 | 1 |
| Immunogenicity of somatic mutations in human gastrointestinal cancers. | Science | 2015 | 9 |
| Immunotherapeutic benefit of α-interferon (IFNalpha;) in survivin2B-derived peptide vaccination for advanced pancreatic cancer patients. | Cancer Sci | 2013 | 1 |
| Long-peptide vaccination with driver gene mutations in p53 and Kras induces cancer mutation-specific effector as well as regulatory T cell responses. | Oncoimmunology | 2018 | 16 |
| mRNA vaccine-induced neoantigen-specific T cell immunity in patients with gastrointestinal cancer. | J Clin Invest | 2020 | 20 |
| Neoantigen screening identifies broad TP53 mutant immunogenicity in patients with epithelial cancers. | J Clin Invest | 2019 | 8 |
| Persistent mutant oncogene specific T cells in two patients benefitting from anti-PD-1. | J Immunother Cancer | 2019 | 2 |
| Phenotypic signatures of circulating neoantigen-reactive CD8+ T cells in patients with metastatic cancers | Cancer Cell | 2023 | 3 |
| Prostatic acid phosphatase as a target molecule in specific immunotherapy for patients with nonprostate adenocarcinoma. | J Immunother | 2005 | 1 |
| Safety and Activity of PolyPEPI1018 Combined with Maintenance Therapy in Metastatic Colorectal Cancer: an Open-Label, Multicenter, Phase Ib Study. | Clin Cancer Res | 2022 | 12 |
| T cell neoepitope discovery in colorectal cancer by high throughput profiling of somatic mutations in expressed genes. | Gut | 2017 | 2 |
结肠癌抗原验证使用的方法清单
每种抗原对T细胞的活化均通过了实验方法验证，其中ELISPOT是验证抗原最多的方法，其次是四聚体荧光染色法判断抗原特异性T细胞的活化数量。
| 检测方法 | 鉴定的抗原数量 |
| 放射性同位素 ⁵¹Cr（铬-51）标记 | 14 |
| ELISA | 21 |
| ELISPOT | 190 |
| IFN-gamma 流式细胞染色 | 6 |
| 四聚体染色 | 42 |
| surface plasmon resonance (SPR) | 5 |
结肠癌抗原结合的HLA分布
肿瘤抗原通过结合HLA呈递，以激活抗肿瘤的T细胞免疫。如下清单列出了结肠癌抗原结合的HLA亚型，使用结肠癌抗原进行肿瘤疫苗开发时，须参考HLA分布特征。
| HLA类型 | 表位数量 |
| HLA-A*02:01 | 57 |
| HLA-B*15:01 | 27 |
| HLA-A*02:07 | 14 |
| HLA-A*03:01 | 13 |
| HLA-A*24:02; HLA-C*07:02 | 12 |
| HLA-B*40:01; HLA-C*05:01 | 5 |
| HLA-DQA1*05:01;HLA-DRB1*13:01;HLA-DRB1*03:01;HLA-B*13:02;HLA-C*08:02;HLA-C*06:02 | 3 |
| HLA-B*38:01;HLA-DQB1*02:02;HLA-B*18:01;HLA-C*01:02;HLA-B*27:05;HLA-A*30:02;HLA-DQA1*02:01;HLA-A*32:01;HLA-DQB1*02:01;HLA-B*14:01;HLA-DQB1*06:02;HLA-B*07:02;HLA-DRB1*07:01;HLA-A*23:01;HLA-DRB1*15:01;HLA-DQA1*01:02;HLA-C*03:04 | 2 |
| HLA-DRB1*04:01;HLA-DRA*01:01;HLA-B*08:01;HLA-B*15:11;HLA-A*68:01;HLA-A*01:01;HLA-DQB1*02:05;HLA-B*56:01;HLA-C*07:01;HLA-DQA1*01:03;HLA-DQB1*06:03;HLA-C*04:01 | 1 |
结肠癌抗原清单
我们已将结肠癌抗原做成了四聚体复合物，抗原清单请见链接，用户可直接购买，用于检测结肠癌抗原特异性T细胞。
