{"id":1489,"date":"2026-01-13T05:56:01","date_gmt":"2026-01-13T05:56:01","guid":{"rendered":"https:\/\/www.guixiong.com.cn\/?page_id=1489"},"modified":"2026-01-17T11:55:38","modified_gmt":"2026-01-17T11:55:38","slug":"%e5%8d%95%e5%85%8b%e9%9a%86tcr%e6%b5%8b%e5%ba%8f","status":"publish","type":"page","link":"https:\/\/www.guixiong.com.cn\/?page_id=1489","title":{"rendered":"\u5355\u514b\u9686TCR\u6d4b\u5e8f"},"content":{"rendered":"<h4>\u4ec0\u4e48\u662f\u5355\u514b\u9686TCR\u6d4b\u5e8f<\/h4>\n<p>\u4ec0\u4e48\u662f\u5355\u514b\u9686\uff1a\u201c\u5355\u514b\u9686\u201d\u6307\u6765\u6e90\u4e8e\u540c\u4e00\u4e2a\u7956\u7ec6\u80de\u7684\u6240\u6709\u5b50\u4ee3\u7ec6\u80de\uff0c\u5b83\u4eec\u5177\u6709\u5b8c\u5168\u76f8\u540c\u7684TCR alpha\u94fe\u548cbeta\u94fe\u5e8f\u5217\uff0c\u8bc6\u522b\u540c\u4e00\u4e2a\u6297\u539f\u8868\u4f4d\u3002<a href=\"https:\/\/www.guixiong.com.cn\/?page_id=84\">\u201cTCR\u7684\u5355\u514b\u9686\u201d\u5728\u4e0d\u540c\u8bed\u5883\u4e0b\u7684\u542b\u4e49<\/a>:<\/p>\n<ol>\n<li>\u79d1\u7814\/\u6cbb\u7597\u5f00\u53d1\u89d2\u5ea6\uff1a\u5206\u79bb\u6216\u5de5\u7a0b\u5316\u7279\u5b9a\u7684\u5355\u514b\u9686TCR\uff0c\u7528\u4e8e\uff1a<\/li>\n<\/ol>\n<ul>\n<li style=\"list-style-type: none;\">\n<ul>\n<li>\u6784\u5efaTCR-T\u7ec6\u80de\u7597\u6cd5\uff08\u5c06\u8bc6\u522b\u80bf\u7624\u6297\u539f\u7684\u5355\u514b\u9686TCR\u57fa\u56e0\u5bfc\u5165\u60a3\u8005T\u7ec6\u80de\uff09\uff1b<\/li>\n<li>\u7814\u7a76\u7279\u5b9a\u81f4\u75c5\u6027T\u7ec6\u80de\u514b\u9686\uff08\u5982\u5728\u81ea\u8eab\u514d\u75ab\u75c5\u4e2d\uff09\u7684\u529f\u80fd\u3002<\/li>\n<\/ul>\n<\/li>\n<\/ul>\n<p>2. \u4ece\u75c5\u7406\u5b66\/\u4e34\u5e8a\u8bca\u65ad\u89d2\u5ea6\uff1a<\/p>\n<ul>\n<li style=\"list-style-type: none;\">\n<ul>\n<li>\u5728T\u7ec6\u80de\u6dcb\u5df4\u7624\u7b49\u75be\u75c5\u4e2d\uff0c\u80bf\u7624\u6027T\u7ec6\u80de\u662f\u7531\u4e00\u4e2a\u5f02\u5e38T\u7ec6\u80de\u6269\u589e\u800c\u6765\uff0c\u5176TCR\u57fa\u56e0\u91cd\u6392\u5448\u73b0\u5355\u514b\u9686\u6027\uff08\u5373\u6240\u6709\u80bf\u7624\u7ec6\u80de\u6709\u76f8\u540c\u7684TCR\u91cd\u6392\u5e8f\u5217\uff09\u3002<\/li>\n<li>\u8fd9\u4e0e\u6b63\u5e38\u591a\u514b\u9686T\u7ec6\u80de\u7fa4\u4f53\uff08TCR\u9ad8\u5ea6\u591a\u6837\uff09\u5f62\u6210\u9c9c\u660e\u5bf9\u6bd4\uff0c\u56e0\u6b64\u68c0\u6d4bTCR\u662f\u5426\u4e3a\u5355\u514b\u9686\u662f\u8bca\u65adT\u7ec6\u80de\u6076\u6027\u80bf\u7624\u7684\u91cd\u8981\u4f9d\u636e\u3002<\/li>\n<\/ul>\n<\/li>\n<\/ul>\n<p>\u5355\u514b\u9686TCR\u6d4b\u5e8f\u5c31\u662f\u5bf9\u6bcf\u79cd\u5355\u514b\u9686T\u7ec6\u80de\u4e2d\u914d\u5bf9TCR alpha\u94fe\u548cbeta\u94fe\u5e8f\u5217\u6d4b\u5b9a\u540e\uff0c\u5c55\u73b0\u51fa\u6784\u6210TCR\u7684\u5b8c\u6574V(D)J-C\u533a\u7684\u6838\u9178\u5e8f\u5217\u3002\u7531\u4e8eTCR\u5e8f\u5217\u5728\u4e2a\u4f53\u4e2d\u7684\u9ad8\u5ea6\u591a\u6837\u6027\uff0c\u83b7\u5f97\u7684TCR\u914d\u5bf9\u7684TCR alpha\u94fe\u548cbeta\u94fe\u5e8f\u5217\u662f\u4e00\u4e2a\u6570\u91cf\u572810^2-10^6\u4e4b\u95f4\u7684\u5e8f\u5217\u6570\u636e\u5e93\u3002\u82e5\u8fd9\u4e9bT\u7ec6\u80de\u662f\u901a\u8fc7<a href=\"https:\/\/www.guixiong.com.cn\/?page_id=133\">pMHC\u591a\u805a\u4f53<\/a>\u5206\u9009\u51fa\u7684\uff0c\u5219\u80fd\u770b\u5230\u7ed3\u5408pMHC\u591a\u805a\u4f53\u6700\u4e3b\u8981\u7684TCR\u514b\u9686(\u5929\u7136TCR alpha\u94fe\u548cbeta\u94fe\u914d\u5bf9)\uff0c\u6b64\u65f6TCR\u7684\u514b\u9686\u79cd\u7c7b\u7ea6\u4e3a10^2-10^3\u4e4b\u95f4\u3002<\/p>\n<h4>\u5355\u514b\u9686TCR\u6d4b\u5e8f\u7684\u57fa\u672c\u539f\u7406<\/h4>\n<p>\u5355\u514b\u9686TCR\u6d4b\u5e8f\u4f7f\u7528\u4e86\u591a\u6a21\u6001\u6280\u672f\u53e0\u52a0\u65b9\u5f0f\u5b8c\u6210\uff0c\u4ee5\u6807\u51c6\u5316\u3001\u6a21\u5757\u5316\u65b9\u5f0f\u7ec4\u5408\u5982\u4e0b\u6280\u672f\uff1a<\/p>\n<ul>\n<li>\u6db2\u6ef4\u751f\u6210\u6280\u672f<\/li>\n<li>\u4e73\u6db2PCR\u6280\u672f<\/li>\n<li>\u5fae\u53cd\u5e94\u4f53\u7cfb\u5185\u878d\u5408\u57fa\u56e0\u6269\u589e\u6280\u672f<\/li>\n<li>NGS\u6587\u5e93\u6784\u5efa\u4e0eUMI\u5f15\u5bfc\u7684\u7247\u6bb5\u62fc\u63a5\u6280\u672f<\/li>\n<\/ul>\n<h4>\u5355\u514b\u9686TCR\u6d4b\u5e8f\u540e\u4f1a\u83b7\u5f97\u4ec0\u4e48\uff1f<\/h4>\n<p>\u5355\u514b\u9686TCR\u6d4b\u5e8f\u6838\u5fc3\u4ef7\u503c\u5728\u4e8e\uff1a\u53ef\u89e3\u6790\u51fa\u76f8\u540c\u514b\u9686T\u7ec6\u80de\u514d\u75ab\u5e94\u7b54\u7684\u7279\u5f02\u6027\u3001\u514b\u9686\u52a8\u6001\uff0c\u5177\u4f53\u5305\u62ec\uff1a<\/p>\n<ul>\n<li>\u6bcf\u4e2aT\u7ec6\u80de\u7684\u5b8c\u6574\u03b1\/\u03b2\u94fe\u5929\u7136\u914d\u5bf9\u4fe1\u606f\uff08\u8fd9\u662f\u8bc6\u522b\u6297\u539f\u7684\u5173\u952e\uff09\n<ul>\n<li style=\"list-style-type: none;\">\n<ul>\n<li>TCR alpha\u94fe\u7684V\u533a\u3001J\u533a\u3001C\u533a\u57fa\u56e0\u540d\u79f0\uff0cCDR3\u533a\u57df\u6838\u9178\u5e8f\u5217\u548c\u6c28\u57fa\u9178\u5e8f\u5217\uff0c\u5e76\u53bb\u9664CDR3\u533a\u57df\u51fa\u73b0\u7ec8\u6b62\u5b50\u7684\u514b\u9686\uff1b<\/li>\n<li>TCR beta\u94fe\u7684V\u533a\u3001D\u533a\u3001J\u533a\u3001C\u533a\u57fa\u56e0\u540d\u79f0\uff0cCDR3\u533a\u57df\u6838\u9178\u5e8f\u5217\u548c\u6c28\u57fa\u9178\u5e8f\u5217\uff0c\u5e76\u53bb\u9664CDR3\u533a\u57df\u51fa\u73b0\u7ec8\u6b62\u5b50\u7684\u514b\u9686\uff1b<\/li>\n<\/ul>\n<\/li>\n<\/ul>\n<\/li>\n<li>\u540c\u4e00\u514b\u9686\uff08\u5373\u62e5\u6709\u76f8\u540cTCR\uff09\u7684T\u7ec6\u80de\u662f\u5426\u6269\u589e\uff0c\u8f93\u51fa\u7ed3\u679c\u662f\u6bcf\u79cd\u514b\u9686\u7684\u514b\u9686\u6570\u91cf\uff0c\u5373\u914d\u5bf9\u7684TCR alpha\u94fe\u548cTCR beta\u94fe\u514b\u9686\u6570\u91cf\u3002\u5728<a href=\"https:\/\/www.guixiong.com.cn\/?page_id=133\">pMHC\u591a\u805a\u4f53<\/a>\u5206\u9009\u7684T\u7ec6\u80de\u4e2d\uff0c\u8be5\u514b\u9686\u6570\u91cf\u4e30\u5ea6\u6700\u9ad8\u7684TCR\u662f\u6297\u539f\u53cd\u5e94\u7684\u4e3b\u8981\u540e\u7eed\u514b\u9686\u3002<\/li>\n<\/ul>\n<h4>\u5355\u514b\u9686TCR\u6d4b\u5e8f\u4e0e\u5176\u4ed6TCR\u6d4b\u5e8f\u6bd4\u8f83<\/h4>\n<ul>\n<li>\u4e0eBulk TCR\u6d4b\u5e8f\u76f8\u6bd4\uff1a\u5355\u514b\u9686TCR\u6d4b\u5e8f\u53ef\u4ee5\u83b7\u5f97\u914d\u5bf9\u7684TCR alpha\u94fe\u548cbeta\u94fe\u5e8f\u5217\uff0c\u8fd9\u4e00\u70b9\u548c\u5355\u7ec6\u80deTCR\u6d4b\u5e8f\u4e00\u6837\u3002\u800cBulk TCR\u6d4b\u5e8f\u65e0\u6cd5\u83b7\u5f97TCR alpha\u94fe\u548cbeta\u94fe\u7684\u914d\u5bf9\u5173\u8054\u4fe1\u606f\uff1b<\/li>\n<li>\u4e0e\u5355\u7ec6\u80deTCR\u6d4b\u5e8f\u6bd4\uff1b\u5355\u514b\u9686TCR\u6d4b\u5e8f\u83b7\u5f97\u7684TCR\u514b\u9686\u6570\u91cf\u7ea7\u662f\u5355\u7ec6\u80deTCR\u6d4b\u5e8f\u768410\u500d\u4ee5\u4e0a\uff0c\u53ef\u4ee5\u5145\u5206\u91c7\u96c6\u6837\u672c\u4e2dT\u7ec6\u80de\u514b\u9686\u5206\u5e03\u7279\u6027\u3002<\/li>\n<\/ul>\n<h4>\u68c0\u6d4b\u670d\u52a1\u6240\u9700\u7528\u6237\u63d0\u4f9b\uff1a<\/h4>\n<ul>\n<li>\u6bcf\u6837\u672c\u6d3bT\u7ec6\u80de\u6570\u91cf &gt;1*105(\u7ea6PBMC&gt;4*105)\uff0c-80\u5ea6\u65e0\u8840\u6e05\u51bb\u5b58\u6db2\u4fdd\u5b58\uff0c\u6216\u6db2\u6c2e\u542b\u8840\u6e05\u51bb\u5b58\u6db2\u4fdd\u5b58\u3002\u51bb\u5b58\u7ec6\u80de\u5230\u8fbe\u6211\u65b9\u7ec6\u80de\u5b9e\u9a8c\u5ba4\u540e\uff0c\u6211\u65b9\u5c06\u590d\u82cf24h\u540e\u5f00\u59cb\u68c0\u6d4b\u3002<\/li>\n<li>\u6297\u51dd\u5168\u8840\u5e38\u6e2948h\u4ee5\u5185(\u542b\u8fd0\u8f93\u65f6\u95f4)\uff0c\u5168\u8840\u6837\u672c\u5230\u8fbe\u6211\u65b9\u7ec6\u80de\u5b9e\u9a8c\u5ba4\u540e\uff0c\u6211\u65b9\u63d0\u53d6PBMC\u540e\u5f00\u59cb\u68c0\u6d4b\u3002<\/li>\n<\/ul>\n<h4>\u4ea4\u4ed8\u7ed3\u679c<\/h4>\n<ul>\n<li>\u6bcf\u4e2a\u6837\u672c\u7684fastq\u539f\u59cb\u6570\u636e(\u6d4b\u5e8f\u6570\u636e\u91cf&gt;50M reads)<\/li>\n<li>\u6bcf\u514b\u9686\u914d\u5bf9\u7684a\u94fe\u548cb\u94fe\uff0c\u5176\u4e2da\u94fe\u542bV-J-C\u533a\u57fa\u56e0\u540d\u79f0+CDR3\u5e8f\u5217\uff0cb\u94fe\u542bV-(D)-J-C\u533a\u57fa\u56e0\u540d\u79f0+CDR3\u5e8f\u5217<\/li>\n<li>\u6bcf\u79cdTCR\u7684\u514b\u9686\u6570<\/li>\n<\/ul>\n<hr \/>\n<h4>\u5355\u514b\u9686TCR\u6d4b\u5e8f\u7684\u5f00\u53d1\u6307\u6807<\/h4>\n<p>\u5b9e\u6d4b\u6a21\u578b\u8bbe\u7f6e\u4e3a\uff1a\u901a\u8fc7\u4e0a\u683718\u4e07\u4e2aT\u7ec6\u80de\uff0c\u91c7\u96c6180\u4e07\u6db2\u6ef4\u4f5c\u4e3a\u6d4b\u8bd5\u8fc7\u7a0b\uff0c\u4f7f\u7528\u89c6\u89c9\u667a\u80fd\u505a\u8d28\u91cf\u76d1\u63a7\uff0c\u5b8c\u6210\u4ee5\u4e0b\u6d4b\u8bd5\uff1a<\/p>\n<blockquote><p>1. \u89c6\u89c9\u667a\u80fd\u4e0b\u6db2\u6ef4\u751f\u6210\u8fc7\u7a0b<br \/>\n<iframe src=\"\/\/player.bilibili.com\/player.html?bvid=BV19Pr6BTEuC&amp;page=1\" frameborder=\"no\" scrolling=\"no\" allowfullscreen=\"allowfullscreen\"><br \/>\nwidth=&#8221;100%&#8221;<br \/>\nheight=&#8221;500&#8243;<br \/>\nscrolling=&#8221;no&#8221;<br \/>\nframeborder=&#8221;0&#8243;<br \/>\nallowfullscreen=&#8221;true&#8221;&gt;<br \/>\n<\/iframe><br \/>\n\u8be5\u89c6\u9891\u663e\u793a\u4e86\u7ec6\u80de\u4e0ePCR\u53cd\u5e94\u4f53\u7cfb\u88ab\u6db2\u6ef4\u5305\u88f9\u65f6\u7684\u5149\u573a\u3001\u8367\u5149\u573a\u89c6\u89c9\u667a\u80fd\u76d1\u63a7\u8fc7\u7a0b\uff0c\u5176\u4e2d\u89c6\u9891\u4e0a\u7aef\u4e3a\u76d1\u63a7\u53c2\u6570\uff0c\u5de6\u4e0b\u4e3a\u6db2\u6ef4\u751f\u6210\u7684\u5149\u573a\u9ad8\u901f\u6444\u5f71\u8bb0\u5f55\uff0c\u53f3\u4e0b\u4e3a\u667a\u80fd\u8bc6\u522b\u5149\u573a\u4e0b\u6db2\u6ef4\u4fa7\u5411\u9762\u79ef\u3001\u5149\u4eae\u5ea6\u3001\u6db2\u6ef4\u6570\u91cf\u5b9e\u6d4b\u66f2\u7ebf\u56fe\u3002<\/p><\/blockquote>\n<p>&nbsp;<\/p>\n<blockquote><p>2.\u6db2\u6ef4\u751f\u6210\u5e73\u5747\u9762\u79ef\u8ba1\u7b97\u3002\u7edf\u8ba1\u6bcf\u7fa4\u9762\u79ef\u8fd1\u4f3c\u7684\u6db2\u6ef4\u6570\u91cf\uff0c\u901a\u8fc7\u67f1\u72b6\u56fe\u5c55\u793a\u4e0d\u540c\u6db2\u6ef4\u4fa7\u5411\u9762\u79ef\u7684\u6570\u91cf\u5dee\u522b\uff0c\u4e0d\u540c\u6db2\u6ef4\u9762\u79ef\u5dee\u8ddd\u8d8a\u5c0f\u3001\u6570\u91cf\u5dee\u522b\u8d8a\u5c0f\u8d8a\u597d\u3002\u4e0b\u56fe\u662f\u7edf\u8ba1\u5355\u6b21\u6db2\u6ef4\u751f\u6210\u65f6\uff0c\u4e0d\u540c\u6db2\u6ef4\u9762\u79ef\u7684\u6db2\u6ef4\u6570\u91cf\u7edf\u8ba1\u56fe\u3002<\/p>\n<p><img fetchpriority=\"high\" decoding=\"async\" class=\"aligncenter wp-image-1496\" style=\"font-weight: bold;\" src=\"https:\/\/guixiong.com.cn\/wp-content\/uploads\/2026\/01\/\u6db2\u6ef4\u9762\u79ef-\u6570\u91cf\u7edf\u8ba1.jpg\" alt=\"\" width=\"600\" height=\"439\" srcset=\"https:\/\/guixiong.com.cn\/wp-content\/uploads\/2026\/01\/\u6db2\u6ef4\u9762\u79ef-\u6570\u91cf\u7edf\u8ba1.jpg 946w, https:\/\/guixiong.com.cn\/wp-content\/uploads\/2026\/01\/\u6db2\u6ef4\u9762\u79ef-\u6570\u91cf\u7edf\u8ba1-300x219.jpg 300w, https:\/\/guixiong.com.cn\/wp-content\/uploads\/2026\/01\/\u6db2\u6ef4\u9762\u79ef-\u6570\u91cf\u7edf\u8ba1-768x562.jpg 768w\" sizes=\"(max-width: 600px) 100vw, 600px\" \/><\/p><\/blockquote>\n<p>&nbsp;<\/p>\n<blockquote><p>3.\u6db2\u6ef4\u751f\u6210\u8fc7\u7a0b\u4e2d\u53cc\u7ec6\u80de\u7387\u3001\u7a7a\u8f7d\u7ec6\u80de\u751f\u6210\u7edf\u8ba1\u3002\u901a\u8fc7FAM\u3001JOE\u8367\u5149\u8ba1\u7b97\u53cc\u7ec6\u80de\u6807\u8bb0\uff0c\u901a\u8fc7FITC\u548cDAPI\u8367\u5149\u8ba1\u7b97\u6db2\u6ef4\u7a7a\u8f7d\u7ec6\u80de\u6bd4\u4f8b\u3002<\/p>\n<p><img decoding=\"async\" class=\"aligncenter wp-image-1495\" src=\"https:\/\/guixiong.com.cn\/wp-content\/uploads\/2026\/01\/\u6db2\u6ef4\u9762\u79ef-\u7a7a\u7ec6\u80de-\u53cc\u7ec6\u80de\u7edf\u8ba1.jpg\" alt=\"\" width=\"600\" height=\"494\" srcset=\"https:\/\/guixiong.com.cn\/wp-content\/uploads\/2026\/01\/\u6db2\u6ef4\u9762\u79ef-\u7a7a\u7ec6\u80de-\u53cc\u7ec6\u80de\u7edf\u8ba1.jpg 835w, https:\/\/guixiong.com.cn\/wp-content\/uploads\/2026\/01\/\u6db2\u6ef4\u9762\u79ef-\u7a7a\u7ec6\u80de-\u53cc\u7ec6\u80de\u7edf\u8ba1-300x247.jpg 300w, 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