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\u7136\u800c\uff0c\u5f53\u7814\u7a76\u8005\u8bd5\u56fe\u5728\u72ec\u7acb\u7684\u3001\u5927\u89c4\u6a21\u7684\u65b0\u51a0\u60a3\u8005\u961f\u5217\uff08\u5982immuneRACE\u9879\u76ee\u4e2d\u7684694\u540d\u60a3\u8005\uff09\u4e2d\u5bfb\u627e\u8fd9\u4e9b\u201c\u7cbe\u786e\u5339\u914d\u201d\u7684TCR\u65f6\uff0c\u5374\u53d1\u73b0\u5b83\u4eec\u7684\u68c0\u51fa\u7387\u6781\u4f4e\u3002\u8fd9\u662f\u56e0\u4e3a\uff1a<\/p>\n
    \n
  • TCR\u7684\u6781\u7aef\u591a\u6837\u6027<\/strong>\uff1a\u5373\u4f7f\u662f\u8bc6\u522b\u540c\u4e00\u6297\u539f\u7684T\u7ec6\u80de\uff0c\u5176TCR\u5e8f\u5217\u4e5f\u53ef\u80fd\u5b58\u5728\u7ec6\u5fae\u4f46\u5173\u952e\u7684\u5dee\u5f02\u3002<\/li>\n
  • \u6d4b\u5e8f\u6df1\u5ea6\u9650\u5236<\/strong>\uff1a\u9ad8\u901a\u91cf\u6d4b\u5e8f\u65e0\u6cd5\u8986\u76d6\u4e2a\u4f53\u5168\u90e8\u7684TCR\u5e93\uff0c\u7a00\u6709\u7684\u514b\u9686\u578b\u5f88\u5bb9\u6613\u88ab\u9057\u6f0f\u3002\u8fd9\u79cd\u201c\u7cbe\u786e\u5339\u914d\u201d\u7b56\u7565\u7684\u4f4e\u7075\u654f\u5ea6\uff0c\u4e25\u91cd\u5236\u7ea6\u4e86TCR\u4f5c\u4e3a\u53ef\u9760\u751f\u7269\u6807\u5fd7\u7269\u7684\u5e94\u7528\u3002<\/li>\n<\/ul>\n
    \u201c\u5143\u514b\u9686\u578b\u201d\u6846\u67b6\u2014\u2014\u7528\u751f\u5316\u76f8\u4f3c\u6027\u4ee3\u66ff\u5e8f\u5217\u540c\u4e00\u6027<\/h3>\n
    \u4e3a\u4e86\u89e3\u51b3\u4e0a\u8ff0\u56f0\u5883\uff0c\u7814\u7a76\u56e2\u961f\u63d0\u51fa\u4e86\u4e00\u4e2a\u4f18\u96c5\u7684\u89e3\u51b3\u65b9\u6848\uff1a\u4e0d\u518d\u6267\u7740\u4e8e\u5bfb\u627e\u5b8c\u5168\u76f8\u540c\u7684TCR\u5e8f\u5217\uff0c\u800c\u662f\u5bfb\u627e\u751f\u5316\u6027\u8d28\u76f8\u4f3c\u7684TCR\u5bb6\u65cf\u3002<\/p>\n
    \u201c\u5143\u514b\u9686\u578b\u201d\u7684\u6784\u5efa\u5305\u542b\u4e09\u4e2a\u6838\u5fc3\u8981\u7d20\uff1a<\/p>\n
    1. \u4e2d\u5fc3TCR \uff08Centroid\uff09<\/strong><\/section>\n
    \u4ece\u6297\u539f\u5bcc\u96c6\u5b9e\u9a8c\uff08\u5982MIRA\uff09\u4e2d\u9009\u53d6\u4e00\u4e2a\u9ad8\u8d28\u91cf\u7684TCR\u5e8f\u5217\u4f5c\u4e3a\u4e2d\u5fc3\u3002<\/section>\n
    2. \u751f\u5316\u8ddd\u79bb\u534a\u5f84 \uff08TCRdist Radius\uff09<\/strong><\/section>\n
    \u4f7f\u7528\u540d\u4e3aTCRdist\u7684\u751f\u5316\u8ddd\u79bb\u5ea6\u91cf\uff08\u8003\u8651\u6c28\u57fa\u9178\u66ff\u6362\u7684BLOSUM62\u77e9\u9635\u548cCDR3\u533a\u57df\u7684\u6743\u91cd\uff09\uff0c\u8ba1\u7b97\u4e00\u4e2a\u6700\u4f18\u534a\u5f84\u3002\u8fd9\u4e2a\u534a\u5f84\u7684\u8bbe\u5b9a\u539f\u5219\u662f\uff1a\u5728\u672a\u63a5\u89e6\u8fc7\u8be5\u6297\u539f\u7684\u80cc\u666fTCR\u5e93\uff08\u5982\u8110\u5e26\u8840\u6216\u5408\u6210\u5e93\uff09\u4e2d\uff0c\u843d\u5165\u6b64\u534a\u5f84\u5185\u7684TCR\u9891\u7387\u4f4e\u4e8e\u767e\u4e07\u5206\u4e4b\u4e00\uff081E-6\uff09\uff0c\u4ece\u800c\u4fdd\u8bc1\u4e86\u7279\u5f02\u6027\u3002<\/section>\n
    3. CDR3\u57fa\u5e8f\u7ea6\u675f \uff08CDR3 Motif\uff09<\/strong>\u4e3a\u4e86\u8fdb\u4e00\u6b65\u63d0\u9ad8\u7279\u5f02\u6027\uff0c\u53ef\u4ee5\u4ece\u534a\u5f84\u5185\u7684\u6297\u539f\u76f8\u5173TCR\u4e2d\u63d0\u53d6\u4e00\u4e2a\u4fdd\u5b88\u7684\u6c28\u57fa\u9178\u5e8f\u5217\u57fa\u5e8f\uff08Motif\uff09\uff0c\u4f5c\u4e3a\u989d\u5916\u7684\u7b5b\u9009\u6761\u4ef6\u3002<\/section>\n
    \u901a\u8fc7\u8fd9\u4e2a\u6846\u67b6\uff0c\u4e00\u4e2a\u539f\u672c\u53ea\u5728\u5c11\u6570\u4e2a\u4f53\u4e2d\u51fa\u73b0\u7684\u201c\u79c1\u6709\u201d\u4e2d\u5fc3TCR\uff0c\u5c31\u53d8\u6210\u4e86\u4e00\u4e2a\u53ef\u4ee5\u5728\u4eba\u7fa4\u4e2d\u5e7f\u6cdb\u68c0\u6d4b\u5230\u7684\u201c\u516c\u5171\u201d\u5143\u514b\u9686\u578b\u3002<\/p>\n
    \"\"<\/p>\n
    \u56fe1\uff1a\u63cf\u8ff0\u4e86\u4e00\u79cd\u7528\u4e8e\u8bc6\u522b\u548c\u91cf\u5316\u6297\u539f\u7279\u5f02\u6027T\u7ec6\u80de\u53d7\u4f53\uff08TCR\uff09\u7684\u8ba1\u7b97\u65b9\u6cd5\uff0c\u79f0\u4e3a\u201cmeta-clonotype\uff08\u5143\u514b\u9686\u578b\uff09\u201d\u6784\u5efa\u4e0e\u5206\u6790\u6d41\u7a0b\u3002<\/p>\n
    \u56fe1A-\u4ece\u6297\u539f\u76f8\u5173\u7684TCR\u4e2d\u5b9a\u4e49 meta-clonotypes\uff0c\u76ee\u6807\u662f\u4e0d\u4ec5\u53ea\u5173\u6ce8\u5355\u4e2aTCR\uff0c\u800c\u662f\u60f3\u627e\u51fa\u4e00\u7fa4\u5728\u751f\u5316\u7279\u6027\u4e0a\u76f8\u4f3c\u7684TCR\uff0c\u5b83\u4eec\u53ef\u80fd\u90fd\u80fd\u8bc6\u522b\u540c\u4e00\u4e2a\u6297\u539f\u2014\u2014\u8fd9\u5c31\u662f\u201cmeta-clonotype\u201d\u3002\u5177\u4f53\u65b9\u6cd5\u662f\uff1a\u4f7f\u7528\u4e00\u4e2a\u53eb tcrdist3 \u7684\u5de5\u5177\uff0c\u8ba1\u7b97\u6bcf\u4e2aTCR\u4e0e\u5176\u4ed6TCR\u4e4b\u95f4\u7684\u201c\u8ddd\u79bb\u201d\uff08TCRdist\uff09\uff0c\u8fd9\u4e2a\u8ddd\u79bb\u57fa\u4e8eTCR\u6c28\u57fa\u9178\u5e8f\u5217\u7684\u76f8\u4f3c\u6027\uff08\u5c24\u5176\u662fCDR3\u533a\u57df\uff0c\u8fd9\u662f\u8bc6\u522b\u6297\u539f\u7684\u5173\u952e\u90e8\u4f4d\uff09\u3002\u5bf9\u4e8e\u6bcf\u4e00\u4e2a\u201c\u4e2d\u5fc3TCR\u201d\uff08centroid TCR\uff09\uff0c\u5c1d\u8bd5\u4e0d\u540c\u7684\u201c\u534a\u5f84\u201d\uff08radius\uff09\uff0c\u770b\u5728\u8fd9\u4e2a\u534a\u5f84\u5185\u80fd\u5305\u542b\u591a\u5c11\u771f\u5b9e\u7684\u6297\u539f\u76f8\u5173TCR\uff08\u9ed1\u8272\u66f2\u7ebf\uff09\u548c\u5408\u6210\u7684\u80cc\u666fTCR\uff08\u7d2b\u8272\u66f2\u7ebf\uff09\u2014\u2014\u8fd9\u4e9b\u662f\u968f\u673a\u751f\u6210\u7684\u3001\u4e0d\u9488\u5bf9\u8be5\u6297\u539f\u7684TCR\uff0c\u7528\u6765\u6a21\u62df\u201c\u5076\u7136\u51fa\u73b0\u201d\u7684\u60c5\u51b5\u3002<\/p>\n
    \u56fe\u4e2d\u5c55\u793a\u4e86\u4e00\u4e2a\u9488\u5bf9SARS-CoV-2\u75c5\u6bd2\u4e00\u6bb5\u80bd\uff08ORF1ab 1316\u20131330\uff09\u7684meta-clonotype\uff0c\u5176CDR3\u03b2\u94fe\u7684\u4fdd\u5b88\u57fa\u5e8f\u7528logo\u56fe\u8868\u793a\u3002\u7b80\u5355\u8bf4\uff1ameta-clonotype = \u4e00\u4e2a\u4e2d\u5fc3TCR + \u4e00\u4e2a\u6700\u5927\u5b89\u5168\u534a\u5f84\uff08\u6392\u9664\u968f\u673a\u566a\u97f3\uff09+ \u4e00\u4e2aCDR3\u4fdd\u5b88\u57fa\u5e8f\uff0c\u4ee3\u8868\u4e00\u7fa4\u529f\u80fd\u76f8\u4f3c\u7684\u6297\u539f\u7279\u5f02\u6027TCR\u3002<\/p>\n
    \u56fe1B\uff1a\u5728\u5927\u91cfTCR\u6570\u636e\u4e2d\u91cf\u5316\u7b26\u5408meta-clonotype\u7684TCR<\/p>\n
    \u6709\u4e86meta-clonotype\u7684\u5b9a\u4e49\u540e\uff0c\u5c31\u53ef\u4ee5\u5728\u666e\u901a\u4eba\u7684TCR\u6d4b\u5e8f\u6570\u636e\uff08bulk repertoire\uff09\u4e2d\u641c\u7d22\u7c7b\u4f3c\u7684TCR\uff1a<\/p>\n