{"id":84,"date":"2024-07-31T03:28:19","date_gmt":"2024-07-31T03:28:19","guid":{"rendered":"http:\/\/60.204.213.123\/wordpress\/?page_id=84"},"modified":"2024-07-31T10:25:52","modified_gmt":"2024-07-31T10:25:52","slug":"tcr%e6%b5%8b%e5%ba%8f%e7%9a%84%e6%84%8f%e4%b9%89","status":"publish","type":"page","link":"https:\/\/www.guixiong.com.cn\/?page_id=84","title":{"rendered":"TCR\u6d4b\u5e8f\u7684\u610f\u4e49"},"content":{"rendered":"\n<p><strong>TCR\u6d4b\u5e8f\uff0c\u53d1\u73b0T\u7ec6\u80de\u53d7\u4f53\u7ed3\u6784\u548c\u514b\u9686\u5206\u5e03\u662f\u8fd15\u5e74\u91cd\u8981\u7684\u7814\u7a76\u70ed\u70b9<\/strong>\u3002TCR\uff0c\u4e5f\u79f0\u4e3aT\u7ec6\u80de\u53d7\u4f53\uff0c\u662fT\u7ec6\u80de\u8bc6\u522b\u6297\u539f\uff0c\u89e6\u53d1T\u7ec6\u80de\u6fc0\u6d3b\u548c\u589e\u6b96\u7684\u6838\u5fc3\u6a21\u5757\u3002T\u7ec6\u80de\u53d7\u4f53\u76f8\u5173\u8bba\u6587\u6570\u91cf\u6bcf\u5e74\u4ee510%\u7684\u6307\u6570\u589e\u52a0\uff0c\u8fd9\u53ef\u80fd\u4e0e\u514d\u75ab\u7597\u6cd5\u7684\u5927\u91cf\u4e34\u5e8a\u5e94\u7528\u5bc6\u5207\u76f8\u5173\u3002\u65b0\u7684\u514d\u75ab\u6cbb\u7597\u65b9\u6cd5\u3001\u514d\u75ab\u6cbb\u7597\u9776\u70b9\u3001\u514d\u75ab\u6cbb\u7597\u6216\u635f\u4f24\u673a\u5236\uff0c\u90fd\u9700\u8981\u901a\u8fc7TCR\u4f5c\u4e3a\u76f4\u63a5\u6216\u95f4\u63a5\u7684\u7814\u7a76\u8bc1\u636e\u3002<\/p>\n\n\n\n<figure class=\"wp-block-image\"><img decoding=\"async\" src=\"http:\/\/www.haoshengmed.com\/wp-content\/uploads\/2022\/04\/TCR%E6%A3%80%E6%B5%8B1.jpg\" alt=\"TCR\u6d4b\u5e8f\u6280\u672f\u5e94\u7528\u533b\u5b66\u7684\u6210\u679c\u53d1\u8868\u589e\u91cf\u3002\" class=\"wp-image-679\"\/><figcaption class=\"wp-element-caption\"><a href=\"https:\/\/pubmed.ncbi.nlm.nih.gov\/?term=%28TCR%20repertoire%29%20OR%20%28TCR%20Sequencing%29&amp;sort=date&amp;ac=yes\" target=\"_blank\" rel=\"noreferrer noopener\">TCR\u6d4b\u5e8f\u6280\u672f\u5e94\u7528\u533b\u5b66\u7684\u6210\u679c\u53d1\u8868\u589e\u91cf<\/a>\u3002\u6570\u5b57\u6765\u81ea\u4e8ePubmed\uff0c\u56fe\u7247\u6743\u5229\u5f52\u5c5e\u4e8e\u6842\u828e\u533b\u7597<\/figcaption><\/figure>\n\n\n\n<h3 class=\"wp-block-heading\"><strong>TCR\u6d4b\u5e8f\u7684\u7814\u7a76\u548c\u5e94\u7528\u4ef7\u503c<\/strong><\/h3>\n\n\n\n<p>TCR\u68c0\u6d4b\u7684\u4e3b\u8981\u4e09\u4e2a\u7814\u7a76\u4efb\u52a1\u662f\uff1a<\/p>\n\n\n\n<p>1\u3001\u75be\u75c5\u72b6\u6001\u4e0b\uff0c\u6216\u6cbb\u7597\u540e\uff0cTCR\u514b\u9686\u5206\u5e03\u7684\u7279\u5f81\uff0c\u514b\u9686\u5206\u5e03\u72b6\u51b5\u662f\u6bd4\u8f83\u96c6\u4e2d\u7684\uff0c\u8fd8\u662f\u6bd4\u8f83\u5206\u6563\u7684\u3002\u6bd4\u5982\uff0cTCR\u514d\u75ab\u7ec4\u5e93\u6d4b\u5e8f\u6700\u91cd\u8981\u7684\u5e94\u7528\u5b9e\u8df5\u662fCAR-T\u7ec6\u80de\u6cbb\u7597\u524d\u540e\uff0cTCR\u514b\u9686\u5206\u5e03\u7531\u6cbb\u7597\u524d\u7684\u67d0\u4e00\u514b\u9686\u4e3a\u4e3b\uff0c\u53d8\u4e3a\u6cbb\u7597\u540e\u7684\u514b\u9686\u5206\u6563\u5206\u5e03\u3002\u901a\u8fc7\u514b\u9686\u5206\u5e03\u7684\u79bb\u6563\u6027\uff0c\u4ece\u800c\u5224\u65ad\u76f8\u5e94\u80bf\u7624\u6297\u539f\u7279\u5f02\u7ec6\u80de\u51cf\u5c11\u3002<\/p>\n\n\n\n<p>2\u3001\u6297\u539f\u7279\u5f02\u6027TCR\u7684DNA\/RNA\/\u86cb\u767d\u8d28\u7ed3\u6784\u7279\u5f81\uff0c\u5b9a\u4f4dTCR\u7ed3\u6784\u662f\u4e3a\u4e86\u62ff\u5230\u6297\u539f\u7279\u5f02\u6027TCR\u7684\u86cb\u767d\u5e8f\u5217\uff0c\u7528\u4e8e\u6539\u9020T\u7ec6\u80de\uff0c\u4f7f\u66f4\u591a\u7684T\u7ec6\u80de\u53ef\u4ee5\u9776\u5411\u6740\u4f24\u6297\u539f\u7ec6\u80de\u3002<\/p>\n\n\n\n<p>3\u3001\u75be\u75c5\u5206\u578b\uff0c\u68c0\u6d4bTCR\u4e3b\u8981\u7528\u4e8eT\u7ec6\u80de\u6dcb\u5df4\u7624\u548cT\u6dcb\u5df4\u7ec6\u80de\u6027\u767d\u8840\u75c5\u4e2d\u5224\u65ad\u80bf\u7624\u5206\u578b\uff0c\u5373TCR\u91cd\u6392\u68c0\u6d4b\u3002<\/p>\n\n\n\n<h3 class=\"wp-block-heading\"><strong>TCR\u6d4b\u5e8f\u540e\uff0c\u7ed3\u6784\u548c\u514b\u9686\u5206\u5e03\u7684\u68c0\u6d4b\u65b9\u6cd5<\/strong><\/h3>\n\n\n\n<p>\u4ece\u68c0\u6d4b\u6280\u672f\u7684\u5e94\u7528\u89d2\u5ea6\u770b\uff0cTCR\u68c0\u6d4b\u4e3b\u8981\u5206\u4e3a3\u79cd\u65b9\u6cd5\uff1a<\/p>\n\n\n\n<h3 class=\"wp-block-heading\">PCR+\u6bdb\u7ec6\u7ba1\u7535\u6cf3\u7247\u6bb5\u6cd5\u5206\u6790TCR<\/h3>\n\n\n\n<p>\u8fd9\u79cd\u65b9\u6cd5\u4e3b\u8981\u7528\u4e8e\u5224\u65ad\u6076\u6027\u75be\u75c5(\u5982\u6076\u6027\u6dcb\u5df4\u7624\u6216\u6dcb\u5df4\u7ec6\u80de\u6027\u767d\u8840\u75c5)\u662f\u7531\u4e8e\u5728 TCR \u57fa\u56e0\u91cd\u6392\u8fc7\u7a0b\u7684\u4efb\u4f55\u4e00\u4e2a\u9636\u6bb5\u4ea7\u751f\u4e86\u5355\u514b\u9686\u589e\u751f\u800c\u5f15\u53d1\u7684\u75c5\u53d8\uff0c\u5bf9\u6076\u6027\u6dcb\u5df4\u589e\u6b96\u6027\u75be\u75c5\u53ef\u65e9\u671f\u8bca\u65ad\u3001\u4f30\u8ba1\u60a3\u8005\u9884\u540e\u3001\u5224\u65ad\u7597\u6548\u3001\u76d1\u6d4b\u590d\u53d1\uff0c\u4e00\u822c\u9700\u8981EDTA\u6297\u51dd\u7684\u9aa8\u9ad3\u6216\u5168\u8840\u6807\u672c\u3002<\/p>\n\n\n\n<h3 class=\"wp-block-heading\">Bulk TCR\u6d4b\u5e8f\u6cd5<\/h3>\n\n\n\n<p>\u4e3b\u8981\u5206\u4e3a5\u2019RACE\u6cd5\u7684\u5168\u957f\u6d4b\u5e8f\u548c\u591a\u91cdPCR\u6d4b\u5e8f\u3002\u524d\u8005\u80fd\u591f\u83b7\u77e5TCR\u5e8f\u5217\u7684\u5b8c\u6574\u7ed3\u6784\uff0c\u5bf9\u4e8e\u5b8c\u6574\u8d28\u91cf\u7684\u6807\u672c\u6765\u8bf4\uff0c5\u2019RACE\u6cd5\u83b7\u5f97\u7684TCR\u5b8c\u6574\u7ed3\u6784\u53ef\u4ee5\u7528\u4e8e\u83b7\u5f97\u5bf9\u5e94\u86cb\u767d\u5e8f\u5217\uff0c\u4f46\u662f\u9047\u5230RNA\u76843\u2018\u7aef\u4e22\u5931\uff0cTCR\u4fe1\u606f\u4f1a\u6709\u5f88\u5927\u7f3a\u9677\uff1b\u5bf9\u4e8e\u540e\u8005\u6765\u8bf4\uff0c\u591a\u91cdPCR\u6cd5\u4f1a\u8bbe\u8ba1V\u533a\u7684CDR3\u7684\u7279\u5f02\u6027\u6269\u589e\u5f15\u7269\uff0c\u5373\u4fbf\u662f\u4e22\u5931RNA 3\u2019\u7aef\uff0c\u4e5f\u53ef\u4ee5\u83b7\u5f97CDR3\u533a\u57df\u7684\u4fe1\u606f\uff0c\u8fd9\u5bf9\u4e8e\u5206\u6790TCR\u7684\u514b\u9686\u5206\u5e03\u975e\u5e38\u6709\u5229\u3002<\/p>\n\n\n\n<h4 class=\"wp-block-heading\">\u5355\u7ec6\u80deTCR\u6d4b\u5e8f<\/h4>\n\n\n\n<p>\u80fd\u591f\u83b7\u5f97\u6bcf\u4e2aT\u7ec6\u80de\u7684TCR\u5e8f\u5217\u548c\u5176\u4ed6\u8f6c\u5f55\u672c\u5e8f\u5217\uff0c\u5176\u4e2dTCR\u5e8f\u5217\u662f\u5168\u957fTCR\u7ed3\u6784\uff0c\u6d4b\u5e8f\u65b9\u6cd5\u57285\u2019RACE\u6cd5\u57fa\u7840\u4e0a\u589e\u52a0\u4e86\u6a21\u677f\u8f6c\u6362\u548c\u5de2\u5f0fPCR\u6280\u672f\u3002\u5355\u7ec6\u80deTCR\u6d4b\u5e8f\u7684\u5f3a\u5927\u4ef7\u503c\u5728\u4e8e\uff0c\u4e0d\u4f46\u53ef\u4ee5\u83b7\u5f97\u5168\u957fTCR\u7ed3\u6784\uff0c\u8fd8\u80fd\u83b7\u5f97\u7ec6\u80de\u5206\u7fa4\u7279\u5f02\u6027\u7684TCR\u5206\u5e03\uff0c\u6bd4\u5982CD4+ Treg \u7ec6\u80de\u7684TCR\u514b\u9686\u5206\u5e03\u7279\u6027\uff0cCD8+ CTL\u7ec6\u80de\u7684TCR\u514b\u9686\u5206\u5e03\u7279\u6027\u3002\u9488\u5bf9\u6027\u5206\u6790TCR\u4e3b\u514b\u9686\u7ec6\u80de\u7684CD\u5206\u578b\u4ef7\u503c\u5728\u4e8e\uff1a\u8be5\u4fe1\u606f\u53ef\u4ee5\u5224\u65ad\u6297\u539f\u7279\u5f02\u6027T\u7ec6\u80de\u4e3b\u8981\u53d1\u6325\u7684\u4f5c\u7528\uff0c\u662f\u8f85\u52a9\u8c03\u8282\u4f5c\u7528\uff0c\u8fd8\u662f\u7ec6\u80de\u6740\u4f24\u4f5c\u7528\uff0c\u6216\u8005\u66f4\u591a\u7684\u5176\u4ed6T\u7ec6\u80de\u529f\u80fd\u3002\u66f4\u91cd\u8981\u7684\u662f\uff0c\u5355\u7ec6\u80deTCR\u6d4b\u5e8f\u53ef\u4ee5\u540c\u65f6\u83b7\u5f97\u540c\u4e00\u4e2aT\u7ec6\u80de\u914d\u5bf9\u7684TRCA\u548cTRCB\u5e8f\u5217\u7ed3\u6784\uff0c2021\u5e74Science\u53d1\u8868\u8bba\u6587\u8bc1\u5b9e\uff0c\u6b63\u786e\u7684TRCB\u4e0epMHC\u7ed3\u5408\u4f4d\u7f6e\u624d\u4f1a\u89e6\u53d1T\u7ec6\u80de\u6fc0\u6d3b\uff0c\u800cTRCA\u548cTRCB\u7684\u6b63\u786e\u914d\u5bf9\u51b3\u5b9a\u4e86TCR\u4e0epMHC\u7684\u6b63\u786e\u7ed3\u5408\u3002<\/p>\n\n\n\n<figure class=\"wp-block-image\"><img decoding=\"async\" src=\"http:\/\/www.haoshengmed.com\/wp-content\/uploads\/2022\/04\/TCR%E6%A3%80%E6%B5%8B-1024x572.jpg\" alt=\"\u4e0d\u540c\u7684TCR\u7ed3\u6784\u5bfc\u81f4\u4e0d\u540c\u7684\u514d\u75ab\u54cd\u5e94\uff0cTCR\u5bf9\u514d\u75ab\u54cd\u5e94\u7684\u6709\u6548\u6027\u4e0e\u7ed3\u6784\u7684\u7a7a\u95f4\u6784\u8c61\u5bc6\u5207\u5173\u8054\" class=\"wp-image-680\"\/><figcaption class=\"wp-element-caption\">\u4e0d\u540c\u7684TCR\u7ed3\u6784\u5bfc\u81f4\u4e0d\u540c\u7684\u514d\u75ab\u54cd\u5e94\uff0cTCR\u5bf9\u514d\u75ab\u54cd\u5e94\u7684\u6709\u6548\u6027\u4e0e\u7ed3\u6784\u7684\u7a7a\u95f4\u6784\u8c61\u5bc6\u5207\u5173\u8054<\/figcaption><\/figure>\n\n\n\n<h3 class=\"wp-block-heading\"><strong>\u603b\u7ed3<\/strong><\/h3>\n\n\n\n<p>\u6240\u4ee5\uff0c\u4ece\u68c0\u6d4b\u6280\u672f\u8fd4\u56de\u5230TCR\u7684\u5e94\u7528\u6a21\u5f0f\u65b9\u9762\uff1a<\/p>\n\n\n\n<p>1. \u5e94\u7528\u4e8eTCR\u514b\u9686\u591a\u6837\u6027\u68c0\u6d4b\u65f6\uff0c\u76ee\u6807\u662f\u5224\u65adT\u7ec6\u80de\u76f8\u5e94\u6297\u539f\u7684\u591a\u6837\u6027\u7a0b\u5ea6\uff0c\u591a\u91cdPCR\u6cd5\u62165\u2019RACE TCR\u6d4b\u5e8f\u5747\u53ef\u80dc\u4efb\uff1b<\/p>\n\n\n\n<p>2. \u5e94\u7528\u4e8e\u7cbe\u786e\u53d1\u73b0\u6297\u539f\u7279\u5f02\u6027T\u7ec6\u80de\u7684TCR\u7ed3\u6784\uff0c\u4ece\u800c\u9493\u53d6\u6297\u539f\u7279\u5f02\u6027\u9776\u7ec6\u80de\uff0c\u6216\u8005\u6784\u5efa\u9776\u5411\u6297\u539f\u7684TCR-T\u7ec6\u80de\uff0c\u5219\u9700\u89815\u2019RACE TCR\u6d4b\u5e8f\u6216\u5355\u7ec6\u80deTCR\u6d4b\u5e8f\uff1b<\/p>\n\n\n\n<p>3. \u82e5\u5e94\u7528\u4e8e\u8840\u6db2\u80bf\u7624\u4e2dT\u7cfb\u6dcb\u5df4\u7624\u6216\u6dcb\u5df4\u7ec6\u80de\u6027\u767d\u8840\u75c5\u7684\u5206\u578b\u68c0\u6d4b\uff0cPCR+\u6bdb\u7ec6\u7ba1\u7535\u6cf3\u7247\u6bb5\u6cd5\u5206\u6790TCR\u5c31\u53ef\u4ee5\u80dc\u4efb\uff0c\u5f53\u7136\uff0c\u5982\u679c\u8fdb\u884c\u9ad8\u6df1\u5ea6TCR\u6d4b\u5e8f\uff0c\u5219\u53ef\u4ee5\u5e94\u7528\u4e8eT\u7cfb\u8840\u6db2\u80bf\u7624\u7684\u5fae\u5c0f\u6b8b\u7559\u7076\u68c0\u6d4b\u3002<\/p>\n<p>Views: 9<\/p>","protected":false},"excerpt":{"rendered":"<p>TCR\u6d4b\u5e8f\uff0c\u53d1\u73b0T\u7ec6\u80de\u53d7\u4f53\u7ed3\u6784\u548c\u514b\u9686\u5206\u5e03\u662f\u8fd15\u5e74\u91cd\u8981\u7684\u7814\u7a76\u70ed\u70b9\u3002TCR\uff0c\u4e5f\u79f0\u4e3aT\u7ec6\u80de\u53d7\u4f53\uff0c\u662fT\u7ec6\u80de\u8bc6\u522b\u6297\u539f\uff0c\u89e6&#8230;<\/p>\n","protected":false},"author":2,"featured_media":0,"parent":0,"menu_order":0,"comment_status":"closed","ping_status":"closed","template":"","meta":{"_acf_changed":false,"_kadence_starter_templates_imported_post":false,"_kad_post_transparent":"","_kad_post_title":"","_kad_post_layout":"","_kad_post_sidebar_id":"","_kad_post_content_style":"","_kad_post_vertical_padding":"","_kad_post_feature":"","_kad_post_feature_position":"","_kad_post_header":false,"_kad_post_footer":false,"_kad_post_classname":"","footnotes":""},"class_list":["post-84","page","type-page","status-publish","hentry"],"acf":[],"_links":{"self":[{"href":"https:\/\/www.guixiong.com.cn\/index.php?rest_route=\/wp\/v2\/pages\/84","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/www.guixiong.com.cn\/index.php?rest_route=\/wp\/v2\/pages"}],"about":[{"href":"https:\/\/www.guixiong.com.cn\/index.php?rest_route=\/wp\/v2\/types\/page"}],"author":[{"embeddable":true,"href":"https:\/\/www.guixiong.com.cn\/index.php?rest_route=\/wp\/v2\/users\/2"}],"replies":[{"embeddable":true,"href":"https:\/\/www.guixiong.com.cn\/index.php?rest_route=%2Fwp%2Fv2%2Fcomments&post=84"}],"version-history":[{"count":3,"href":"https:\/\/www.guixiong.com.cn\/index.php?rest_route=\/wp\/v2\/pages\/84\/revisions"}],"predecessor-version":[{"id":171,"href":"https:\/\/www.guixiong.com.cn\/index.php?rest_route=\/wp\/v2\/pages\/84\/revisions\/171"}],"wp:attachment":[{"href":"https:\/\/www.guixiong.com.cn\/index.php?rest_route=%2Fwp%2Fv2%2Fmedia&parent=84"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}