{"id":929,"date":"2025-07-09T03:12:15","date_gmt":"2025-07-09T03:12:15","guid":{"rendered":"http:\/\/60.204.213.123\/?page_id=929"},"modified":"2025-07-09T03:17:42","modified_gmt":"2025-07-09T03:17:42","slug":"mart1-27-35-hla-a0201-5","status":"publish","type":"page","link":"https:\/\/www.guixiong.com.cn\/?page_id=929","title":{"rendered":"Protein E7 (49-57) H2Kb \u6d41\u5f0f\u9a8c\u8bc1"},"content":{"rendered":"\n

\u9776\u70b9\u8bf4\u660e<\/h3>\n\n\n\n

Protein E7 (49-57) HPV \u75c5\u6bd216\u7684E7\u86cb\u767d\u7684\u591a\u80bdRAHYNIVTF\uff0c\u5728C57BL\/6\u5c0f\u9f20\u4e2d\u88abH2Kb\u5448\u9012\u3002<\/p>\n\n\n\n

\u96cc\u6027C57BL \/ 6\u5c0f\u9f20\u5355\u72ec\u6216\u4e0eGM-CSF\u3001IL-2\u6216\u4e24\u79cd\u7ec6\u80de\u56e0\u5b50\u8054\u5408\u76ae\u5185\u63a5\u79cd HPV16 E7\u80bd(49-57)\uff0c\u53ef\u8bf1\u5bfc\u66f4\u9ad8\u7684CTL\u548c\u7ec6\u80de\u56e0\u5b50\u91ca\u653e\u53cd\u5e94\uff0c\u4e14\u4e0d\u589e\u52a0Treg\u7ec6\u80de\u4e9a\u7fa4\uff0c\u5bf9\u8377\u7624\u5c0f\u9f20\u5177\u6709\u6301\u4e45\u4fdd\u62a4\u4f5c\u7528\u3002<\/p>\n\n\n\n

DNA\u75ab\u82d7\u662f\u4e00\u79cd\u5f88\u6709\u524d\u9014\u7684\u65b9\u6cd5\uff0c\u53ef\u4ee5\u8bf1\u5bfc\u4f53\u6db2\u548c\u7ec6\u80de\u514d\u75ab\u53cd\u5e94\u3002\u5bf9\u4e8eHPV-16\u76f8\u5173\u75be\u75c5\u7684\u514d\u75ab\u6cbb\u7597\uff0cE7\u86cb\u767d\u88ab\u8ba4\u4e3a\u662f\u4e00\u4e2a\u4e3b\u8981\u7684\u5019\u9009\u8005\uff0c\u56e0\u4e3a\u5b83\u5728\u6240\u6709HPV-16\u9633\u6027\u80bf\u7624\u4e2d\u8868\u8fbe\u3002<\/p>\n\n\n\n

\u9ad8\u5371\u578b\u4eba\u4e73\u5934\u7624\u75c5\u6bd2\u76f8\u5173\u764c\u75c7\u8868\u8fbe\u8bf1\u5bfc\u548c\u7ef4\u6301\u6076\u6027\u8868\u578b\u7684\u75c5\u6bd2\u764c\u86cb\u767d\uff08\u4f8b\u5982\uff0cE6 \u548c E7\uff09\u3002\u8fd9\u4e9b\u86cb\u767d\u8d28\u7684\u75c5\u6bd2\u8d77\u6e90\u4f7f\u5b83\u4eec\u6210\u4e3a\u5f00\u53d1\u6cbb\u7597\u6027\u75ab\u82d7\u7684\u7406\u60f3\u9776\u70b9\u3002\u8bc6\u522b\u6297\u539f\u5448\u9012\u7ec6\u80de\u8868\u9762\u86cb\u767d\u7684\u9a86\u9a7c\u5355\u57df\u6297\u4f53\u7247\u6bb5\uff08\u7eb3\u7c73\u6297\u4f53\u6216VHH\uff09\u53ef\u4ee5\u4f5c\u4e3a\u8fde\u63a5\u5230\u5176\u4e0a\u7684\u6297\u539f\u7684\u76ee\u6807\u8f93\u9001\u8f7d\u4f53\u3002<\/p>\n\n\n\n

HLA\u7c7b\u578b<\/h3>\n\n\n\n

H2kb\u662fC57BL\/6\u7684MHC-I\u4e9a\u578b<\/p>\n\n\n\n

<\/p>\n\n\n\n

\u6297\u539f\u548cHLA\u7684\u4eb2\u548c\u529b<\/h3>\n\n\n\n

<\/p>\n\n\n\n

NetMHCpan 4.1<\/strong> <\/a> \u8ba1\u7b97RAHYNIVTF\u4e0eH2Kb \u4eb2\u548c\u529b\u4e3a 686.47nM\uff0c\u4e3a\u7a33\u5b9a\u7ed3\u5408\u3002<\/p>\n\n\n\n

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\u4ea7\u54c1\u7c7b\u578b\u548c\u5e94\u7528<\/h3>\n\n\n\n

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\u4ea7\u54c1\u7c7b\u578b<\/td>\u89c4\u683c<\/td>\u4ef7\u683c<\/td><\/tr>
\u6d41\u5f0f\u68c0\u6d4b\u4e0e\u5206\u9009<\/td>Protein E7 (49-57) H2Kb PE \/ APC \/ Alexa Fluor 488<\/td>7200\u5143\/50ug, 11800\u5143\/100ug<\/td><\/tr>
\u78c1\u73e0\u5206\u9009<\/td>Protein E7 (49-57) H2Kb\u78c1\u73e0<\/td>8200\u5143\/50ug, 12800\u5143\/100ug<\/td><\/tr>
\u751f\u7269\u7d20\u5316\u590d\u5408\u7269<\/td>Protein E7 (49-57) H2Kb -biotin<\/td>6800\u5143\/50ug, 11400\u5143\/100ug<\/td><\/tr><\/tbody><\/table><\/figure>\n\n\n\n

\u5b9e\u6d4b\u7ed3\u679c<\/h3>\n\n\n\n

<\/p>\n\n\n\n

\u80bd\u523a\u6fc0PBMC\u540e\u68c0\u6d4b\u6297\u539f\u7279\u5f02\u6027CD8+T\u7ec6\u80de<\/h3>\n\n\n\n

RAHYNIVTF\u591a\u80bd\u523a\u6fc0H2Kb \u9650\u5236\u6027PBMC\u540e\uff0c\u4f7f\u7528Protein E7 (49-57)H2Kb \u56db\u805a\u4f53\u548c\u6297CD8-FITC\u6297\u4f53\u68c0\u6d4b\u3002\u4e0b\u56fe\u5de6\u56fe\u4e3a\u6297\u539f\u80bdRAHYNIVTF\u523a\u6fc0\u7ec4\uff0c\u53f3\u56fe\u4e3a\u672a\u52a0\u6297\u539f\u80bd\u523a\u6fc0\u7ec4\u3002<\/p>\n\n\n\n

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\"\"<\/figure>\n<\/div>\n\n\n\n
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\"\"<\/figure>\n<\/div>\n<\/div>\n\n\n\n

\u9776\u70b9\u7ed3\u5408\u7684TCR\u5e8f\u5217<\/h3>\n\n\n\n

\u65e0<\/p>\n\n\n\n

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\u76f8\u5173\u6587\u7ae0<\/h3>\n\n\n\n\n\n\n\t\n\n\t\n\t\n\t\n\t\n\t
Reference - PMID<\/th>Reference - Journal<\/th>Reference - Date<\/th>Reference - Title<\/th>\n<\/tr>\n<\/thead>\n
17689584<\/td>Virology<\/td>2007<\/td>Multiple copies of a tumor epitope in a recombinant hepatitis B surface antigen (HBsAg) vaccine enhance CTL responses, but not tumor protection.<\/td>\n<\/tr>\n
18193057<\/td>Nat Med<\/td>2008<\/td>Targeting of antigen to the herpesvirus entry mediator augments primary adaptive immune responses.<\/td>\n<\/tr>\n
29792298<\/td>Cancer Immunol Res<\/td>2018<\/td>Nanobody-Antigen Conjugates Elicit HPV-Specific Antitumor Immune Responses.<\/td>\n<\/tr>\n
11886264<\/td>Virology<\/td>2002<\/td>Enhanced immunogenicity of HPV 16 E7 fusion proteins in DNA vaccination.<\/td>\n<\/tr>\n
27222537<\/td>Mol Cancer Ther<\/td>2016<\/td>Protection against HPV-16-Associated Tumors Requires the Activation of CD8+ Effector Memory T Cells and the Control of Myeloid-Derived Suppressor Cells.<\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n\n\n\n\n

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Views: 4<\/p>","protected":false},"excerpt":{"rendered":"

\u9776\u70b9\u8bf4\u660e Protein E7 (49-57) HPV \u75c5\u6bd216\u7684E7\u86cb\u767d\u7684\u591a\u80bdRAHYNIVTF\uff0c\u5728C57B…<\/p>\n","protected":false},"author":1,"featured_media":0,"parent":174,"menu_order":0,"comment_status":"closed","ping_status":"closed","template":"","meta":{"_acf_changed":false,"_kadence_starter_templates_imported_post":false,"_kad_post_transparent":"","_kad_post_title":"","_kad_post_layout":"","_kad_post_sidebar_id":"","_kad_post_content_style":"","_kad_post_vertical_padding":"","_kad_post_feature":"","_kad_post_feature_position":"","_kad_post_header":false,"_kad_post_footer":false,"_kad_post_classname":"","footnotes":""},"class_list":["post-929","page","type-page","status-publish","hentry"],"acf":[],"_links":{"self":[{"href":"https:\/\/www.guixiong.com.cn\/index.php?rest_route=\/wp\/v2\/pages\/929","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/www.guixiong.com.cn\/index.php?rest_route=\/wp\/v2\/pages"}],"about":[{"href":"https:\/\/www.guixiong.com.cn\/index.php?rest_route=\/wp\/v2\/types\/page"}],"author":[{"embeddable":true,"href":"https:\/\/www.guixiong.com.cn\/index.php?rest_route=\/wp\/v2\/users\/1"}],"replies":[{"embeddable":true,"href":"https:\/\/www.guixiong.com.cn\/index.php?rest_route=%2Fwp%2Fv2%2Fcomments&post=929"}],"version-history":[{"count":5,"href":"https:\/\/www.guixiong.com.cn\/index.php?rest_route=\/wp\/v2\/pages\/929\/revisions"}],"predecessor-version":[{"id":1053,"href":"https:\/\/www.guixiong.com.cn\/index.php?rest_route=\/wp\/v2\/pages\/929\/revisions\/1053"}],"up":[{"embeddable":true,"href":"https:\/\/www.guixiong.com.cn\/index.php?rest_route=\/wp\/v2\/pages\/174"}],"wp:attachment":[{"href":"https:\/\/www.guixiong.com.cn\/index.php?rest_route=%2Fwp%2Fv2%2Fmedia&parent=929"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}