桂芎生物

靶点说明

ESAT-6 (82-90) 是6 kDa early secretory antigenic target ESXA蛋白的肽段AMASTEGNV，物种是Mycobacterium tuberculosis H37Rv。

AMASTEGNV是一种线性肽表位（表位ID 3064），是结核分枝杆菌中研究的6 kDa早期分泌抗原靶点的一部分（UniProt：P9WNK7）。

HLA类型

HLA-A*02:01， 中国人分布频率12.036%

抗原和HLA的亲和力

NetMHCpan 4.1  计算AMASTEGNV与HLA-A*02:01亲和力为 591.10 nM。

产品类型和应用

产品类型	规格	价格
流式检测与分选	ESAT-6 (82-90) HLA-A*02:01 PE / APC / Alexa Fluor 488	7200元/50ug, 11800元/100ug
磁珠分选	ESAT-6 (82-90) HLA-A*02:01 磁珠	8200元/50ug, 12800元/100ug
生物素化复合物	ESAT-6 (82-90) HLA-A*02:01 -biotin	6800元/50ug, 11400元/100ug

实测结果

肽刺激PBMC后检测抗原特异性CD8+T细胞

AMASTEGNV多肽刺激HLA-A*02:01限制性PBMC后，使用ESAT-6 (82-90) HLA-A*02:01 PE 四聚体和抗CD8-FITC抗体检测。下图左图为抗原肽AMASTEGNV刺激组，右图为未加抗原肽刺激组。

靶点结合的TCR序列

请查看IEDB数据库

相关文章

Reference – PMID	Reference – Journal	Reference – Date	Reference – Title
9419365	Proc Natl Acad Sci U S A	1998	Human cytolytic and interferon gamma-secreting CD8+ T lymphocytes specific for Mycobacterium tuberculosis.
23555576	PLoS One	2013	A broad profile of co-dominant epitopes shapes the peripheral Mycobacterium tuberculosis specific CD8+ T-cell immune response in South African patients with active tuberculosis.
25809751	Int J Infect Dis	2015	Frequency of Mycobacterium tuberculosis-specific CD8+ T-cells in the course of anti-tuberculosis treatment.
25631937	Eur J Immunol	2015	Human CD8 T lymphocytes recognize Mycobacterium tuberculosis antigens presented by HLA-E during active tuberculosis and express type 2 cytokines.
25809750	Int J Infect Dis	2015	Mycobacterium tuberculosis-specific and MHC class I-restricted CD8+ T-cells exhibit a stem cell precursor-like phenotype in patients with active pulmonary tuberculosis.